Operation Procedure of Mouse Interleukin 1 (IL-1) ELISA Kit

Operation Procedure of Mouse Interleukin 1 (IL-1) ELISA Kit

1. Discard the liquid and spin dry. Fill each well with diluted washing liquid, shake for 30 seconds, shake off the washing liquid, pat dry with absorbent paper. Repeat this 5 times, pat dry.

2. Add 50μl of developer A to each well, and then add 50μl of developer B, mix gently, and develop at 37 ° C for 15 minutes in the dark.

3. Remove the enzyme labeling plate and add 50μl of stop solution to each well to stop the reaction (the blue color turns to yellow at this time).

4. Determination: Zero the blank holes and measure the absorbance value (OD value) of each well at 450nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution.

5. Calculate the linear regression equation of the standard curve according to the concentration of the standard product and the corresponding OD value, and then calculate the corresponding sample concentration on the regression equation according to the OD value of the sample. It can also be calculated using various application software. It should be remembered that since the sample is diluted, its actual concentration should be multiplied by the total dilution factor.

"Mouse interleukin 1 (IL-1) ELISA kit exercises" the following related products:

Mouse Interleukin 1β (IL-1β) ELISA Kit Mouse Leukotriene B4 (LTB4) ELISA Kit Mouse Leukemia Inhibitory Factor Receptor (LIFR) ELISA Kit Mouse Epidermal Growth Factor (EGF) ELISA Kit Mouse Intestinal Fatty Acid Binding Protein (iFABP) ELISA Kit Mouse Telomerase (TE) ELISA Kit Mouse Matrix Metalloproteinase 5 (MMP-5) ELISA Kit Mouse Matrix Metalloproteinase 4 (MMP-4) ELISA Kit Small Mouse MMP-9 / Gelatinase B ELISA Kit Mouse MMP-1 (TIMP-1) ELISA Kit Mouse Basic Fibroblast Growth Factor (bFGF) ELISA Kit Mouse Macrophage Inflammatory Protein 5 (MIP-5) ELISA Kit Mouse Soluble E Selectin (sE-selectin) ELISA Kit Mouse Soluble Intercellular Adhesion Molecule 1 (sICAM-1) ELISA Kit Small Mouse soluble vascular cell adhesion molecule 1 (sVCAM-1) ELISA kit mouse immunoglobulin A (IgA) ELISA kit mouse immunoglobulin E (IgE) ELISA kit mouse immunoglobulin G (IgG) ELISA Reagent test kit

The 4+2 seats golf carts our factory produce with gas and battery power, different colors as your requirement are available,the types of our two seat golf carts incluce EZGO,YAMAHA,CLUB CAR, the extra options like rear flip seat,rain cover,,sun shade cover,music player,alloy rim,,ect are all available as your requirements, our 4+2 seats golf carts with many use purpose,like hotel,for personal usage,touring,park,,etc, the most outsanding feature of our 4+2 seats golf carts is high cost performance, the motor we use is 5kw Ac Or 4kw Dc, battery is 150ah,6 cells 8 volt deep cycle storage battery that with the max range over 100kms for each charge,battery lifespan is up to 800 tims recharge cycles, we also accept all kinds of OEM services for our golf carts, welcome for your inquiry for the details!

4+2 Seaters Golf Sightseeing Cars

4+2 Seaters Golf Sightseeing Cars,4+2 Seaters Golf Buggy,4+2 Seaters Electric Golf Buggy

Yongkang Jinghang Sightseeing Vehicle Co., Ltd. , https://www.jhgolfcarts.com