Ensuring the safety of the centrifuge is the top priority, as a loss of control over the centrifugal force can lead to serious damage. It's essential to check that the centrifuge tubes are properly balanced, that the rotational speed does not exceed the set limit, and that the rotor is in good condition without signs of corrosion or wear.
Equipment Required: High-speed refrigerated centrifuge, rotor, and centrifuge tubes.
Separation Technology Operation Steps:
- Balance the centrifuge tubes carefully before use.
- Follow the correct procedure for operating the centrifuge.
- Learn and apply proper maintenance techniques for both the centrifuge and its rotors.
Key Considerations During Use:
- Before using the centrifuge, always complete the registration form with details such as user name, rotor type, speed, and time.
- Ensure the centrifuge tubes are well balanced. Pay close attention to their placement within the rotor to maintain equilibrium. Never operate the centrifuge beyond its maximum speed rating.
- Once the centrifuge reaches the preset speed, it's important to step away and monitor from a safe distance. If any unusual noise or vibration occurs, stop the machine immediately.
- Listen for normal operation sounds and observe the centrifuge for any excessive vibration, which could indicate an issue.
- After working with high-salt samples like ammonium sulfate, thoroughly clean the rotor and the centrifuge chamber to prevent residue buildup.
- Ultra-high-speed centrifuges require special training due to their complexity and high rotational speeds. Always receive proper instruction before use.
Step-by-Step Usage Guide:
Develop the habit of registering your use of the centrifuge each time. This helps track usage and ensures accountability.
- Set the desired temperature (usually 4°C), then place the rotor into the centrifuge chamber. Make sure the rotor is securely clamped onto the shaft. Close the door and allow sufficient time for the chamber to cool down before starting the process.
- Prepare the sample by placing it in a suitable centrifuge tube. Balance the tube using a balance scale. Ensure the tube is tightly sealed and covered. Note: Fill the tube only up to 70% capacity, as excessive filling may cause leakage under strong centrifugal force.
- Place the balanced tubes symmetrically in the rotor. Ensure the rotor cover is fully tightened. If the cover is not properly secured, it may come off during operation, leading to severe damage.
- Close the centrifuge door and adjust the speed and time settings on the control panel according to your requirements.
Always remain attentive during the entire process and follow all safety guidelines to ensure a smooth and secure operation. Regular maintenance and careful handling are crucial for the longevity and performance of the equipment.
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