Precautions for using microplate reader in ELISA experiment

Kamaishu (Shanghai) Biotechnology introduces the precautions for the use of microplate reader

The microplate reader is a precise optical instrument, so a good working environment can not only ensure its accuracy and stability, but also extend its service life.

a. The instrument should be placed in a position without strong magnetic field and interference voltage.

b. The instrument should be placed in an environment with a noise below 40 dB.

c. In order to delay the aging of optical components, direct sunlight should be avoided.

d. The operating ambient temperature should be between 15 ° C and 40 ° C, and the ambient humidity is between 15% and 85%.

e. The voltage should be stable during operation.

f. The operating environment air is clean to avoid water vapor and smoke.

g. Maintain a dry, clean and level work surface, and sufficient operating space.

Operation notes:

a. Use a pipette to add liquid, the pipette tip cannot be mixed.

b. The washing plate should be clean to avoid cross contamination.

c. Strictly follow the instructions of the kit, and the reaction time is accurate.

d. Do not spill samples or reagents on the surface or inside of the instrument. Do the cleaning work after the operation is completed.

e. Do not turn off the power during the measurement.

f. For the deviation of the measurement result caused by the problem of the kit, the parameters should be modified in time according to the actual situation to achieve the best effect.

g. Cover the dust cover after use. In case of technical failure, please contact the manufacturer in time, and do not disassemble the microplate reader without authorization.

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